6 Jun 2025
Relative Evaluation of the Application of Polystyrene Microspheres and Polystyrene Carboxyl Microspheres in Biotechnology – Concentrating On Nucleic Acid Removal.
(LNJNbio Polystyrene Microspheres)
In the field of contemporary biotechnology, microsphere products are extensively utilized in the removal and purification of DNA and RNA due to their high particular area, great chemical security and functionalized surface area homes. Amongst them, polystyrene (PS) microspheres and their acquired polystyrene carboxyl (CPS) microspheres are just one of both most extensively studied and used products. This write-up is supplied with technological assistance and information analysis by Shanghai Lingjun Biotechnology Co., Ltd., intending to systematically contrast the performance differences of these 2 types of materials in the process of nucleic acid removal, covering vital indications such as their physicochemical residential properties, surface alteration capacity, binding efficiency and healing price, and highlight their applicable circumstances through speculative information.
Polystyrene microspheres are uniform polymer bits polymerized from styrene monomers with excellent thermal stability and mechanical strength. Its surface is a non-polar framework and normally does not have active functional teams. As a result, when it is directly made use of for nucleic acid binding, it needs to rely upon electrostatic adsorption or hydrophobic action for molecular addiction. Polystyrene carboxyl microspheres introduce carboxyl practical teams (– COOH) on the basis of PS microspheres, making their surface area capable of additional chemical coupling. These carboxyl teams can be covalently bound to nucleic acid probes, healthy proteins or various other ligands with amino teams via activation systems such as EDC/NHS, thus attaining extra steady molecular fixation. Consequently, from a structural perspective, CPS microspheres have a lot more advantages in functionalization potential.
Nucleic acid removal generally includes steps such as cell lysis, nucleic acid release, nucleic acid binding to solid phase service providers, washing to eliminate contaminations and eluting target nucleic acids. In this system, microspheres play a core role as strong phase service providers. PS microspheres mostly rely on electrostatic adsorption and hydrogen bonding to bind nucleic acids, and their binding performance is about 60 ~ 70%, however the elution performance is reduced, only 40 ~ 50%. On the other hand, CPS microspheres can not only use electrostatic effects yet also accomplish more solid fixation with covalent bonding, lowering the loss of nucleic acids throughout the washing process. Its binding effectiveness can get to 85 ~ 95%, and the elution effectiveness is additionally enhanced to 70 ~ 80%. Additionally, CPS microspheres are also significantly much better than PS microspheres in terms of anti-interference capacity and reusability.
In order to verify the efficiency distinctions in between both microspheres in actual operation, Shanghai Lingjun Biotechnology Co., Ltd. conducted RNA removal experiments. The experimental examples were derived from HEK293 cells. After pretreatment with standard Tris-HCl barrier and proteinase K, 5 mg/mL PS and CPS microspheres were used for removal. The results showed that the typical RNA return drawn out by PS microspheres was 85 ng/ μL, the A260/A280 proportion was 1.82, and the RIN worth was 7.2, while the RNA yield of CPS microspheres was raised to 132 ng/ μL, the A260/A280 ratio was close to the suitable value of 1.91, and the RIN worth reached 8.1. Although the operation time of CPS microspheres is somewhat longer (28 mins vs. 25 mins) and the expense is higher (28 yuan vs. 18 yuan/time), its removal high quality is considerably improved, and it is better for high-sensitivity detection, such as qPCR and RNA-seq.
( SEM of LNJNbio Polystyrene Microspheres)
From the viewpoint of application scenarios, PS microspheres are suitable for massive screening projects and preliminary enrichment with low demands for binding specificity because of their low cost and basic procedure. However, their nucleic acid binding ability is weak and conveniently influenced by salt ion concentration, making them inappropriate for lasting storage or repeated usage. In contrast, CPS microspheres are suitable for trace sample extraction as a result of their abundant surface functional teams, which assist in additional functionalization and can be made use of to create magnetic grain discovery packages and automated nucleic acid removal systems. Although its preparation procedure is reasonably complex and the cost is reasonably high, it reveals stronger flexibility in clinical study and medical applications with rigorous requirements on nucleic acid removal effectiveness and purity.
With the rapid development of molecular diagnosis, genetics editing, liquid biopsy and various other fields, higher demands are positioned on the effectiveness, purity and automation of nucleic acid removal. Polystyrene carboxyl microspheres are slowly changing typical PS microspheres because of their excellent binding efficiency and functionalizable attributes, coming to be the core selection of a new generation of nucleic acid extraction products. Shanghai Lingjun Biotechnology Co., Ltd. is likewise constantly enhancing the fragment dimension circulation, surface area density and functionalization efficiency of CPS microspheres and creating matching magnetic composite microsphere items to fulfill the demands of clinical medical diagnosis, scientific research organizations and industrial clients for top notch nucleic acid extraction services.
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Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding and more. We not only provide products but can also undertake OEM, ODM, and other needs. If you need dna preparation, please feel free to contact us at sales01@lingjunbio.com.
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